![]() When choosing the right pre-stained protein ladder, the most important choice is based on the size of your protein of interest. Prestained ladders also can use highlight bands of a different color, to make it easier to identify different size bands. Protein ladders are most convenient to use when they are supplied ready to use, with no heating, dilution, or addition of reducing agent required before loading onto the gel. Unstained protein ladders are more accurate for sizing proteins, as the dyes used in prestained ladders can slightly distort the apparent size of the protein ladder proteins on the gel. However, an unstained protein ladder can only be visualized following staining with Coomassie or a similar non-specific protein stain. Using an unstained protein ladder is very useful when you need to accurately calculate the size of your protein. calculate the approximate size of your protein by overlaying an image of the membrane with the ladder with the image generated by antibody staining. verify transfer efficiency between gel and membrane (PVDF, nylon, or nitrocellulose) monitor protein separation during SDS-polyacrylamide gel electrophoresis Using a prestained protein ladder when running a western blot helps when you need to:
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